Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Neurons)
Specification
Retina, Neurons
Permeabilization
Yes - Triton X
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: bsa blocking Solution
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
No specific staining at 3 and 4dpf. Tris-HCl and Sodium Citrate antigen retrieval were tested.
Abcam response
Thank you for your valuable feedback. ab40796 has not been previously tested in zebra fish tissue and your data suggests that ab40796 may not be suitable to detect 200 kDa Neurofilament in zebra fish under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 29 2014