Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Ab31457 (1:500) staining Neurofascin in Immunohistochemistry paraffin-embedded Mouse (C57 Black strain) Cerebellum tissue.
Submitted composite image shows indistinct molecular layer fibre staining of the cerebellum and also clear nuclear positivity in many nuclei of the mouse cerebellar Granule cell layer and also the Hippocampus.
Immunostaining this specimen with lower dilution factors increased the nuclear positivity but did not increase the positivity in areas of the brain that gave excellent selective positivity in rat and marmoset using the same antibody.
Submitted composite image shows indistinct molecular layer fibre staining of the cerebellum and also clear nuclear positivity in many nuclei of the mouse cerebellar Granule cell layer and also the Hippocampus.
Immunostaining this specimen with lower dilution factors increased the nuclear positivity but did not increase the positivity in areas of the brain that gave excellent selective positivity in rat and marmoset using the same antibody.
Abcam response
This antibody has been successfully used for IHC-P (ab31457 stained Rat / Marmoset Cerebellum). From your data, however, it is clear that not all protocols will be equally suitable for this technique and that the results obtained can be vastly dependent on the method used. Thank you very much for your review. We hope that this will be helpful for other customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Mar 05 2010