重组Anti-Neurocan抗体[EPR6397] (ab125021)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6397] to Neurocan
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Neurocan抗体[EPR6397]
参阅全部 Neurocan 一抗 -
描述
兔单克隆抗体[EPR6397] to Neurocan -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human Neurocan aa 1-100. The exact sequence is proprietary.
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阳性对照
- Human hippocampus and hypothalamus lysates; Human brain tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.20 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6397 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125021于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 130, 270 kDa (predicted molecular weight: 143 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 130, 270 kDa (predicted molecular weight: 143 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. -
组织特异性
Brain. -
序列相似性
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 1463 Human
- Omim: 600826 Human
- SwissProt: O14594 Human
- Unigene: 169047 Human
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别名
- Chondroitin sulfate proteoglycan 3 antibody
- Cspg3 antibody
- Ncan antibody
see all
图片
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All lanes : Anti-Neurocan antibody [EPR6397] (ab125021) at 1/10000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human hypothalamus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 143 kDa
Observed band size: 130,270 kDa why is the actual band size different from the predicted? -
ab125021, at 1/250 dilution, staining Neurocan in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab125021 被引用在 2 文献中.
- Su H et al. TMAO Aggregates Neurological Damage Following Ischemic Stroke by Promoting Reactive Astrocytosis and Glial Scar Formation via the Smurf2/ALK5 Axis. Front Cell Neurosci 15:569424 (2021). PubMed: 33815059
- Silver DJ et al. Chondroitin sulfate proteoglycans potently inhibit invasion and serve as a central organizer of the brain tumor microenvironment. J Neurosci 33:15603-17 (2013). Human . PubMed: 24068827