Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (differentiated mouse ES cell (D3 line))
Specification
differentiated mouse ES cell (D3 line)
Fixative
Paraformaldehyde
Permeabilization
Yes - in block solution
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (see above)
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse IgG2a alexa fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Mouse ES cells were differentiated for 14 days in serum containing media without LIF in suspension culture conditions. Positive Neuro D1 staining was observed (green).
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提交于 Oct 19 2010