Alexa Fluor® 488荧光Anti-Nestin抗体[Rat-401] (ab197495)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [Rat-401] to Nestin
- Suitable for: ICC/IF
- Reacts with: Rat
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
Alexa Fluor® 488荧光Anti-Nestin抗体[Rat-401]
参阅全部 Nestin 一抗 -
描述
Alexa Fluor® 488荧光小鼠单克隆抗体[Rat-401] to Nestin -
宿主
Mouse -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Rat -
免疫原
Tissue, cells or virus corresponding to Rat Nestin. Homogenized spinal cord tissue from embryonic day 15 (E15) rats.
-
阳性对照
- ICC/IF: PC12 cells.
-
常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
Rat-401 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab197495于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100.
|
说明 |
---|
ICC/IF
1/100. |
靶标
-
功能
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
组织特异性
CNS stem cells. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
翻译后修饰
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
-
数据库链接
- SwissProt: P21263 Rat
- Unigene: 9701 Rat
-
别名
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
图片
-
ab197495 staining Nestin in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab197495 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab197495 被引用在 3 文献中.
- Moreno-Bravo JA et al. Uncoupling axon guidance and neuronal migration in Robo3-deficient inferior olivary neurons. J Comp Neurol 530:2868-2880 (2022). PubMed: 35811330
- Moreno-Bravo JA et al. Commissural neurons transgress the CNS/PNS boundary in absence of ventricular zone-derived netrin 1. Development 145:N/A (2018). PubMed: 29343638
- Dominici C et al. Floor-plate-derived netrin-1 is dispensable for commissural axon guidance. Nature 545:350-354 (2017). PubMed: 28445456