Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 2.5% milk in PBS-Tween20
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa 647-conjugated goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Detection
Detection method
fluorescence scanning
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 150, 140 kDa Non-specific: 37, 30 kDa
Additional data
Additional Notes
The secondary antibody (Alexa647-conjugated) was detected using a fluorescence scanner equipped with a red laser (633 nm), at a PMT of 700 V.
The antibody detected specific nestin bands at high molecular weights, but it also recognized potential degradation products of nestin at lower molecular weights.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 27 2012