Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (corneal endothelium)
Specification
corneal endothelium
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 2% BSA, 2% goat serum
Secondary antibody
Name
Non-Abcam antibody was used: alexa fluor 555 goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/500
Additional data
PubMed ID
22949402: View
Additional Notes
Nestin is expressed in extreme peripheral endothelium of human corneas
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Zhiguo He
Verified customer
提交于 Nov 01 2012