Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain and kidney)
Specification
Brain and kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
The images submitted show that, in my hands, this Ab does give good Nestin positivity but that there is also non-specific positvity that I am unable to get rid of without also losing specific signal.
Coloured arrowheads indicate features:
In the upper image of the lateral ventricle and associated svz, nests of cells are strongly positive (red). The ependymal cells (blue) are equally positive. There is also a visible background and nuclear positivity that does disappear on further dilutiion: the specific staining also diminishes significantly.
In the lower image of kidney, there is good, strong immunostaining of podocytes covering the glomerulus. There is equally strong positivity within a significant subpopulation of collecting tubule lining cells, the latter also showing a slight cytoplasmic and brush border positivity.
Coloured arrowheads indicate features:
In the upper image of the lateral ventricle and associated svz, nests of cells are strongly positive (red). The ependymal cells (blue) are equally positive. There is also a visible background and nuclear positivity that does disappear on further dilutiion: the specific staining also diminishes significantly.
In the lower image of kidney, there is good, strong immunostaining of podocytes covering the glomerulus. There is equally strong positivity within a significant subpopulation of collecting tubule lining cells, the latter also showing a slight cytoplasmic and brush border positivity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Dec 13 2010