Application
Flow Cytometry
Sample
Human Cell (Neural Progenitor cells (from human ES cells))
Specification
Neural Progenitor cells (from human ES cells)
Preparation
Cell harvesting/tissue preparation method: Cell Dissociation Buffer
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation
Paraformaldehyde
Permeabilization
Yes - 70% Methanol (ice cold)
Gating Strategy
FSC vs SSC on negative control sample
Other product details
Dilution
1/100
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: FACS buffer (see above)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti mouse IgG1-PE conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/100
Additional data
Additional Notes
Cells were harvested using cell dissociation buffer, fixed in 1% PFA for 10 mins (RT) and then in70% methanol for 30 mins (ice cold). A standard protocol was used from this point. The purple profile shows negative control (mouse IgG) vs green profile (Nestin) a significant shift shows that this antibody is excellent for use in flow cytometry applications.
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提交于 Jan 03 2012