Application
Flow Cytometry
Sample
Mouse Cell (Embryonic stem cells)
Specification
Embryonic stem cells
Preparation
Cell harvesting/tissue preparation method: Trypsination
Sample buffer: PBS, 4%FCS
Sample buffer: PBS, 4%FCS
Permeabilization
No
Gating Strategy
Debris was gated away by FSC/SSC and dead cells by PI.
Other product details
Dilution
1/100
Incubation time
20 minute(s) · Temperature: 4°C · Diluent: PBS, 4% FCS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa flour 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/400
Additional data
Additional Notes
Black line is Nectin 3 (ab16913). Blue line is 2nd antibody only, used as a background control.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 09 2011