Anti-Nav1.7抗体[N68/6] (ab85015)
Key features and details
- Mouse monoclonal [N68/6] to Nav1.7
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
概述
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产品名称
Anti-Nav1.7抗体[N68/6]
参阅全部 Nav1.7 一抗 -
描述
小鼠单克隆抗体[N68/6] to Nav1.7 -
宿主
Mouse -
特异性
Some customers have used this antibody successfully in rat, however our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.
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经测试应用
适用于: IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse
预测可用于: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Mouse dorsal root ganglion tissue; Mouse back skin tissue.
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常规说明
The clone number has been updated from S68-6 to N68/6, both clone numbers name the same antibody clone.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
N68/6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85015于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:7720699, PubMed:17167479, PubMed:25240195, PubMed:26680203, PubMed:15385606, PubMed:16988069, PubMed:17145499, PubMed:19369487, PubMed:24311784). It is a tetrodotoxin-sensitive Na(+) channel isoform (PubMed:7720699). Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:17167479, PubMed:17145499, PubMed:19369487, PubMed:24311784). -
组织特异性
Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion. -
疾病相关
Primary erythermalgia
Indifference to pain, congenital, autosomal recessive
Paroxysmal extreme pain disorder
Generalized epilepsy with febrile seizures plus 7
Febrile seizures, familial, 3B -
序列相似性
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
Contains 1 IQ domain. -
结构域
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
翻译后修饰
Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.
Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4. -
细胞定位
Cell membrane. Cell projection. In neurite terminals. - Information by UniProt
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数据库链接
- Entrez Gene: 6335 Human
- Entrez Gene: 20274 Mouse
- Entrez Gene: 78956 Rat
- Omim: 603415 Human
- SwissProt: Q15858 Human
- SwissProt: Q62205 Mouse
- SwissProt: O08562 Rat
- Unigene: 439145 Human
see all -
别名
- ETHA antibody
- GEFSP7 antibody
- hNE Na antibody
see all
图片
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IHC image of Nav1.7 staining in mouse dorsal root ganglion formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85015, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ab85015 at 1/100 dilution staining Nav1.7 in mouse back skin tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used. Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. A Fluorophore conjugated goat anti mouse at 1/50 dilution was used as secondary.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab85015 被引用在 9 文献中.
- Gonzales J et al. Bimodal Imaging of Mouse Peripheral Nerves with Chlorin Tracers. Mol Pharm 18:940-951 (2021). PubMed: 33404254
- Tian JJ et al. Upregulation of Nav1.7 by endogenous hydrogen sulfide contributes to maintenance of neuropathic pain. Int J Mol Med 46:782-794 (2020). PubMed: 32468069
- Jia Q et al. Activating Sirt1 by resveratrol suppresses Nav1.7 expression in DRG through miR-182 and alleviates neuropathic pain in rats. Channels (Austin) 14:69-78 (2020). PubMed: 32089065
- Gonzales J et al. Fluorescence labeling of a NaV1.7-targeted peptide for near-infrared nerve visualization. EJNMMI Res 10:49 (2020). PubMed: 32409881
- Gonzales J et al. Fluorescence Imaging of Peripheral Nerves by a Nav1.7-Targeted Inhibitor Cystine Knot Peptide. Bioconjug Chem 30:2879-2888 (2019). PubMed: 31647222
- Xie MX et al. Nuclear Factor-kappaB Gates Nav1.7 Channels in DRG Neurons via Protein-Protein Interaction. iScience 19:623-633 (2019). PubMed: 31446225
- Hofmann L et al. Characterization of small fiber pathology in a mouse model of Fabry disease. Elife 7:N/A (2018). PubMed: 30328411
- Bi RY et al. Estradiol upregulates voltage-gated sodium channel 1.7 in trigeminal ganglion contributing to hyperalgesia of inflamed TMJ. PLoS One 12:e0178589 (2017). IHC-Fr ; Rat . PubMed: 28582470
- Bi RY et al. Involvement of trigeminal ganglionic Nav 1.7 in hyperalgesia of inflamed temporomandibular joint is dependent on ERK1/2 phosphorylation of glial cells in rats. Eur J Pain 17:983-94 (2013). PubMed: 23242737