Anti-NUP214抗体(ab70497)
Key features and details
- Rabbit polyclonal to NUP214
- Suitable for: ICC, ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NUP214抗体
参阅全部 NUP214 一抗 -
描述
兔多克隆抗体to NUP214 -
宿主
Rabbit -
特异性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: ICC, ICC/IF, IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residues 1250 and 1300 of human NUP214. (NP_005076.3)
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阳性对照
- WB: HeLa and KEK293T lysate; IP: HeLa lysate; IHC: ovarian carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 19339552
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 214 kDa).
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IP | (1) |
Use at 2-10 µg/mg of lysate.
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说明 |
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ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 19339552 |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 214 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex. -
组织特异性
Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development. -
疾病相关
Note=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene.
Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET. -
结构域
Contains FG repeats. -
翻译后修饰
Probably glycosylated as it reacts with wheat germ agglutinin (WGA). -
细胞定位
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
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数据库链接
- Entrez Gene: 8021 Human
- Entrez Gene: 227720 Mouse
- Omim: 114350 Human
- SwissProt: P35658 Human
- SwissProt: Q80U93 Mouse
- Unigene: 654530 Human
- Unigene: 289686 Mouse
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别名
- 214 kDa nucleoporin antibody
- CAIN antibody
- CAN antibody
see all
图片
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All lanes : Anti-NUP214 antibody (ab70497) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293T whole cell lysate at 50 µg
Predicted band size: 214 kDa -
NUP214 was immunoprecipitated from HeLa whole cell lysate (0.1 mg per IP reaction, 20% loaded) with ab70497 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70497 at 0.04 µg/mL.
Lane 1: ab70497 IP in HeLa whole cell lysate.Lane 2: ab70497 IP in HeLa whole cell lysate.
Lane 2: rabbit anti-NUP214 antibody IP in HeLa whole cell lysate.
Lane 4: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling NUP214 with ab70497 at 1/200 dilution.Detection: DAB
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling NUP214 with ab70497 at 1/200 dilution.Detection: DAB
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab70497 被引用在 18 文献中.
- Cigdem S et al. SET-NUP214 and MLL cooperatively regulate the promoter activity of the HoxA10 gene. Genes Cells 26:830-837 (2021). PubMed: 34320268
- Senbas Akyazi B et al. Interaction of influenza A virus NS2/NEP protein with the amino-terminal part of Nup214. Turk J Biol 44:82-92 (2020). PubMed: 32256144
- Lv L et al. Long Non-coding RNA LINC00114 Facilitates Colorectal Cancer Development Through EZH2/DNMT1-Induced miR-133b Suppression. Front Oncol 9:1383 (2019). PubMed: 31921641
- Ben-Yishay R et al. Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore. J Cell Biol 218:2962-2981 (2019). PubMed: 31375530
- Fichtman B et al. Pathogenic Variants in NUP214 Cause "Plugged" Nuclear Pore Channels and Acute Febrile Encephalopathy. Am J Hum Genet 105:48-64 (2019). PubMed: 31178128