Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin equivalent)
Specification
Skin equivalent
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako EnVision Kit 5007
Permeabilization
No
Blocking step
(agent) for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/20
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 3% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Dako EnVision Kit 5007
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
Thank you for your feedback on ab4147. This customer was contacted for further follow up on the negative review of this antibody. Since the product is being used in a tested species and application, it is covered by our Abpromise and subject to a replacement or credit.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 13 2012