Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung,Kidney & Spleen extracts)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
100 µg
Specification
Lung,Kidney & Spleen extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T+5% milk
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-mouse IgG HRP linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 97 kDa
Positive control
Lung and spleen tissue lysates
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 17 2013