Application
Western blot
Sample
Mouse Tissue lysate - whole (liver, muscle)
Loading amount
20 µg
Specification
liver, muscle
Gel Running Conditions
Reduced Denaturing (4-12% BioRad Criterion XT)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS+0,1%Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Jackson AffiniPure #111-035-003
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 second(s)
Bands
Specific: - kDa Non-specific: 48, 70, 80, 105 in m kDa
Additional data
Additional Notes
Tris/SDS lysis buffer, Hybond C extra (GE Healthcare) membrane
Bio-Rad Precision Plus Protein all blue standard (5 ul/well)
Lane 1-2) human WI-38 whole cell lysate (20 ug/well) untreated
Lane 3-5) ZF rat liver lysate (20 ug/well) untreated
Lane 6-8) ZF rat muscle lysate (20 ug/well) untreated
Lane 9-11) CBA mouse liver lysate (20 ug/well) untreated
Lane 12-14) CBA mouse muscle lysate (20 ug/well) untreated
Bio-Rad Precision Plus Protein all blue standard (5 ul/well)
Lane 1-2) human WI-38 whole cell lysate (20 ug/well) untreated
Lane 3-5) ZF rat liver lysate (20 ug/well) untreated
Lane 6-8) ZF rat muscle lysate (20 ug/well) untreated
Lane 9-11) CBA mouse liver lysate (20 ug/well) untreated
Lane 12-14) CBA mouse muscle lysate (20 ug/well) untreated
Abcam response
This negative Abreview is currently being investigated by Abcam Technical Support. Please check here for an update response from Abcam.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 26 2009