Anti-NQO1抗体(ab34173)
Key features and details
- Rabbit polyclonal to NQO1
- Suitable for: ICC, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-NQO1抗体
参阅全部 NQO1 一抗 -
描述
兔多克隆抗体to NQO1 -
宿主
Rabbit -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Guinea pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type HAP1 whole cell lysate. HepG2 and MCF7 whole cell lysate. Human Kidney Whole Tissue Lysate. Recombinant human NQO1 protein. ICC: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34173于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 1 - 5 µg/ml.
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WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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说明 |
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ICC
Use a concentration of 1 - 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
靶标
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功能
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
序列相似性
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1728 Human
- Entrez Gene: 18104 Mouse
- Entrez Gene: 24314 Rat
- Omim: 125860 Human
- SwissProt: Q8CHK7 Guinea pig
- SwissProt: P15559 Human
- SwissProt: Q64669 Mouse
- SwissProt: P05982 Rat
see all -
别名
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: NQO1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab34173 observed at 31 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab34173 was shown to specifically react with NQO1 in wild-type HAP1 cells as signal was lost in NQO1 knockout cells. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. ab34173 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-NQO1 antibody (ab34173) at 1 µg/ml + Human Kidney Whole Tissue Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa -
ab34173 staining NQO1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab34173 at 1 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (151)
ab34173 被引用在 151 文献中.
- Koch M et al. Quantitative Proteomics Identifies Reduced NRF2 Activity and Mitochondrial Dysfunction in Atopic Dermatitis. J Invest Dermatol 143:220-231.e7 (2023). PubMed: 36108803
- Chen H et al. Design and characterization of a heterobifunctional degrader of KEAP1. Redox Biol 59:102552 (2023). PubMed: 36473314
- Lohakul J et al. Mitochondria-Targeted Hydrogen Sulfide Delivery Molecules Protect Against UVA-Induced Photoaging in Human Dermal Fibroblasts, and in Mouse Skin In Vivo. Antioxid Redox Signal 36:1268-1288 (2022). PubMed: 34235951
- Jiwaji Z et al. Reactive astrocytes acquire neuroprotective as well as deleterious signatures in response to Tau and Aß pathology. Nat Commun 13:135 (2022). PubMed: 35013236
- Shi YL et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796