Application
Immunoprecipitation
Immuno-precipitation step
Other - Gamma-bind plus protein A/G (half/half)
Sample
Human Cell lysate - whole cell (KERATINOCYTES, Hacat B)
Specification
KERATINOCYTES, Hacat B
Treatment
inhibitor of nox 50´M
Total protein in input
1000 µg
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: triton,HEPES, NaCl, KCl,EDT plus milk 1%
Dilution
1/100
Additional data
Additional Notes
This antibody is not efficient for immunoprecipitation nor wertern blot.
It worked on the first utilisation, but even after aliquoting it didn't worked for the others.
Abcam response
We are sorry that this antibody did not work for this customer. Customer participate in the AbTrial program for the testing in IP and therefore received a discount code to be used on their nex Abcam order.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. houssam raad
Verified customer
提交于 Mar 12 2015