Application
Western blot
Sample
Human Cell lysate - whole cell (U87 and U373 Human glioblastoma cell lines)
Loading amount
8000 cells
Specification
U87 and U373 Human glioblastoma cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/2500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti Mouse IgG HRP-Linked antibody (Cell signallin
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 26 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Desire Ngoga
Verified customer
提交于 Jun 26 2012