重组Anti-NMDAR2A抗体[EPR2465(2)] (ab124913)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2465(2)] to NMDAR2A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-NMDAR2A抗体[EPR2465(2)]
参阅全部 NMDAR2A 一抗 -
描述
兔单克隆抗体[EPR2465(2)] to NMDAR2A -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human NMDAR2A. The exact sequence is proprietary.
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阳性对照
- WB: Mouse, rat and human brain tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 7.10 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2465(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124913于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 165 kDa.
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
说明 |
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WB
1/1000. Predicted molecular weight: 165 kDa. We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). |
靶标
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功能
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2903 Human
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- Omim: 138253 Human
- SwissProt: Q12879 Human
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 411472 Human
see all -
别名
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
图片
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Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/20000 dilution (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (unpurified)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 165 kDa -
All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/2000 dilution
Lane 1 : Human brain lysates prepared in RIPA lysis method
Lane 2 : Human brain lysates prepared in 1%SDS Hot lysis method
Lane 3 : Mouse brain lysates prepared in RIPA lysis method
Lane 4 : Mouse brain lysates prepared in 1%SDS Hot lysis method
Lane 5 : Rat brain lysates prepared in RIPA lysis method
Lane 6 : Rat brain lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 165 kDa
Observed band size: 165 kDaExposure time
Lanes 1 to 2: 40 seconds
Lanes 3 to 6: 20 secondsWe recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
We are unsure how to define the extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (37)
ab124913 被引用在 37 文献中.
- Zhang SQ et al. Repeated vagus nerve stimulation produces anxiolytic effects via upregulation of AMPAR function in centrolateral amygdala of male rats. Neurobiol Stress 18:100453 (2022). PubMed: 35685681
- Whittsette AL et al. The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors. iScience 25:104754 (2022). PubMed: 35938049
- Wu Q et al. High-frequency repetitive transcranial magnetic stimulation improves spatial episodic learning and memory performance by regulating brain plasticity in healthy rats. Front Neurosci 16:974940 (2022). PubMed: 35992904
- López-Pedrajas R et al. Role of hippocampal NF-κB and GluN2B in the memory acquisition impairment of experiences gathered prior to cocaine administration in rats. Sci Rep 11:20033 (2021). PubMed: 34625609
- Huang L et al. Upregulated NMDAR-mediated GABAergic transmission underlies autistic-like deficits in Htr3a knockout mice. Theranostics 11:9296-9310 (2021). PubMed: 34646371