NIH/3T3核extract裂解物(ab14874)
概述
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产品名称
NIH/3T3核extract裂解物
参阅全部 NIH 3T3 细胞裂解液 -
常规说明
Cell line: NIH/3T3 (Mouse embryonic fibroblast).
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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研究领域
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背景
NIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab14874 尚未被引用在任何文献中。