重组Anti-proNGF抗体[EP1320Y] (ab52918)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1320Y] to proNGF
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-proNGF抗体[EP1320Y]
参阅全部 proNGF 一抗 -
描述
兔单克隆抗体[EP1320Y] to proNGF -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal brain, cerebellum, HeLa, MCF-7, and MDA-MB-231 cell lysates. Mouse brain, cerebellum, and thyroid cell lysates; Rat brain, cerebellum, and thyroid cell lysates. IHC-P: Human brain, human cerebral cortex. ICC/IF: U87-MG cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1320Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52918于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/300.
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WB | (1) |
1/1000. Predicted molecular weight: 27 kDa.
For unpurified, use 1/500. |
IHC-P | (3) |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
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ICC/IF
1/300. |
WB
1/1000. Predicted molecular weight: 27 kDa. For unpurified, use 1/500. |
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
疾病相关
Neuropathy, hereditary sensory and autonomic, 5 -
序列相似性
Belongs to the NGF-beta family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4803 Human
- SwissProt: P01138 Human
- SwissProt: P01139 Mouse
- SwissProt: P25427 Rat
- Unigene: 2561 Human
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别名
- Beta-nerve growth factor antibody
- Beta-NGF antibody
- NGF antibody
see all
图片
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All lanes : Anti-proNGF antibody [EP1320Y] (ab52918) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human cerebellum lysate
Lane 4 : Mouse cerebellum lysate
Lane 5 : Rat cerebellum lysate
Lane 6 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 7 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 8 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsThe positive cell lines (Lanes 7-9) are consistent with the literature: PMID:12944907, Fig1.
Blocking buffer: 5% NFDM/TBST.
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All lanes : Anti-proNGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified)
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal thymus lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Anti-proNGF antibody [EP1320Y] (ab52918) at 1/1000 dilution (purified) + Mouse thyroid lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Anti-proNGF antibody [EP1320Y] (ab52918) at 1/2000 dilution (purified) + Rat thyroid lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52918 at a working dilution of 1 in 250. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of U87-MG cells with purified ab52918 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab52918 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
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Anti-proNGF antibody [EP1320Y] (ab52918) at 1/500 dilution (unpurified) + fetal thyroid tissue at 10 µg
Secondary
Goat anti rabbit IgG HRP antibody at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa -
Immunohistochemical staining of paraffin embedded human brain using unpurified ab52918 at 1/50-1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical staining of proNGF mouse tissue sections (formalin/ PFA-fixed paraffin-embedded tissue sections) with unpurified ab52918. The sections were formaldehyde fixed, subjected to heat mediated antigen retrieval and blocked for 10 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 25°C. An HRP polymer anti-rabbit IgG system was used undiluted, as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (76)
ab52918 被引用在 76 文献中.
- Ye T et al. Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity. Bioact Mater 21:194-208 (2023). PubMed: 36101856
- Sun X et al. Bone marrow mesenchymal stem cells and exercise restore motor function following spinal cord injury by activating PI3K/AKT/mTOR pathway. Neural Regen Res 18:1067-1075 (2023). PubMed: 36254995
- Peng T et al. Nerve Growth Factor (NGF) Encourages the Neuroinvasive Potential of Pancreatic Cancer Cells by Activating the Warburg Effect and Promoting Tumor Derived Exosomal miRNA-21 Expression. Oxid Med Cell Longev 2022:8445093 (2022). PubMed: 36285300
- Lin C et al. S1P promotes corneal trigeminal neuron differentiation and corneal nerve repair via upregulating nerve growth factor expression in a mouse model. Open Life Sci 17:1324-1332 (2022). PubMed: 36313859
- Deng J et al. Extracellular matrix and dermal nerve growth factor dysregulation in prurigo nodularis compared to atopic dermatitis. Front Med (Lausanne) 9:1022889 (2022). PubMed: 36619628