Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (Gel 8%)
Sample
Mouse Cell lysate - whole cell (WT & TRAF2 KO embryonic fibroblasts and macrophage)
Specification
WT & TRAF2 KO embryonic fibroblasts and macrophage
Treatment
TNFα, BV6, LPS and PAM-CSK4 for 24hrs
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk PBST (0.05%)
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Mouse Anti-rabbit IgG (L27A9) mAb (HRP Conjugate)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: 100 kDa Non-specific: 0 kDa
Exposure
3 second(s)
Negative control
Untreated cells - lane 1
MM = Mouse macrophages untreated and LPS treated to induce processing of p100 to p52. (last two lanes)
Positive control
TNFa, BV6, LPS and PAM-CSK4, all of which should induce processing of p100 to p52.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Anna McCormick
Verified customer
提交于 Oct 10 2013