概述

  • 产品名称
  • 描述
    兔多克隆抗体to NFYB
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 16-46 of Human NFYB (NP_006157.1).

  • 阳性对照
    • A549 cell line lysates.

性能

应用

Our Abpromise guarantee covers the use of ab123384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 23 kDa.
ICC/IF 1/10 - 1/50.

靶标

图片

  • Immunofluorescence of HeLa cells labelling NFYB with ab123384. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab123384 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFYB immunoreactivity is localized to the nucleus.

  • Anti-NFYB antibody (ab123384) at 1/100 dilution + A549 cell line lysates at 35 µg

    Predicted band size : 23 kDa

文献

ab123384 has not yet been referenced specifically in any publications.

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