Anti-NFKBIL2抗体(ab101898)
Key features and details
- Rabbit polyclonal to NFKBIL2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NFKBIL2抗体
参阅全部 NFKBIL2 一抗 -
描述
兔多克隆抗体to NFKBIL2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Dog, Leaf frog -
免疫原
Synthetic peptide corresponding to Human NFKBIL2 aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab117409) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101898于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa). |
靶标
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功能
Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold. -
组织特异性
Expressed in heart, skeletal muscle and tracheal epithelial cells. -
序列相似性
Belongs to the Tonsoku family.
Contains 3 ANK repeats.
Contains 7 LRR (leucine-rich) repeats.
Contains 8 TPR repeats. -
结构域
The ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L. -
细胞定位
Cytoplasm. Nucleus. Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks. - Information by UniProt
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数据库链接
- Entrez Gene: 505102 Cow
- Entrez Gene: 475127 Dog
- Entrez Gene: 4796 Human
- Entrez Gene: 72749 Mouse
- Entrez Gene: 366953 Rat
- Omim: 604546 Human
- SwissProt: Q0P5G1 Cow
- SwissProt: Q96HA7 Human
see all -
别名
- I kappa B related protein antibody
- I-kappa-B-related protein antibody
- IkappaBR antibody
see all
图片
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All lanes : Anti-NFKBIL2 antibody (ab101898) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 151 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa, 67 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Ab101898 detects a band at approximately 170-kDa. While this differs to its predicted molecular weight of 151-kDa, this band has been observed in the literature (PMID:21055983).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab101898 被引用在 2 文献中.
- Huang TH et al. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells. Mol Cell 69:879-892.e5 (2018). PubMed: 29478807
- Saredi G et al. H4K20me0 marks post-replicative chromatin and recruits the TONSL–MMS22L DNA repair complex. Nature 534:714-718 (2016). WB . PubMed: 27338793