Application
ChIP
Sample
Human Cell lysate - nuclear (Primary Human T cells)
Specification
Primary Human T cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 4 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37% Formaldehyde
Duration of cross-linking step: 4 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37% Formaldehyde
Detection step
Real-time PCR
Positive control
Primary Human Regulatory T cells stimulated for 24 hours and analysed for NFAT recruitment to a predicted NFAT binding site
Negative control
Primary Human T effector cells stimulated for 24 hours and analysed for NFAT recruitment to the same NFAT binding site. Additionally, an AFM intronic region was also used as an additional negative control.
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Abcam response
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Abcam user community
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提交于 Jun 20 2012