Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreas)
Permeabilization
Yes - tween 20
Specification
pancreas
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Rhodamine
Host species: Goat
Clonality: Polyclonal
Conjugation: Rhodamine
Additional data
Additional Notes
I have stained pancreas tissue embedded in paraffin and stained it with ab86299. Paraffin embedded slides show perfect NF-kB activation specific to islets with no acinar staining while staining the frozen slides with ab86299 stains all over the tissue. I have repeated the staining several times with different concentration of primary,secondary and blocking solution(10% FBS vs goat) but did not get a reliable result in frozen slides comparable to what I get in paraffin embedded slides.
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested with frozen sections and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 21 2017