Anti-NET1抗体(ab113202)
Key features and details
- Rabbit polyclonal to NET1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NET1抗体
参阅全部 NET1 一抗 -
描述
兔多克隆抗体to NET1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla -
免疫原
Synthetic peptide, corresponding to a region within amino acids 546-596 of Human NET1 (NP_001040625).
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阳性对照
- WB: Jurkat whole cell lysate and HEK293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113202于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 62 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 62 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. -
组织特异性
Widely expressed. -
序列相似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10276 Human
- SwissProt: Q7Z628 Human
- Unigene: 25155 Human
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别名
- ARHG8_HUMAN antibody
- ARHGEF8 antibody
- GLYT2 antibody
see all
图片
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Immunoprecipitation of NET1 (6 µg of antibody per mg of HEK-293T cell lysate; 20% of the IP loaded). Anti-Net 1 antibody (ab113202) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 seconds.
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All lanes : Anti-NET1 antibody (ab113202) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 62 kDa
Exposure time: 30 secondsDetected by chemiluminescence.
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Immunoprecipitation of NET1 (6 µg of antibody per mg of Jurkat cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-Net1 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with ab113202; Lane 3- Immunoprecipitation with IgG control. Anti-Net 1 antibody (ab113202) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 3 min.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab113202 被引用在 2 文献中.
- Zhao F et al. Baicalin suppresses lung cancer growth phenotypes via miR-340-5p/NET1 axis. Bioengineered 12:1699-1707 (2021). PubMed: 33955315
- Liang X et al. VTIQ evaluates antitumor effects of NET-1 siRNA by UTMD in HCC xenograft models. Oncol Lett 16:2893-2902 (2018). PubMed: 30127877