Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Non-reduced Denaturing (6%)
Sample
Human Cell lysate - whole cell (PC3)
Specification
PC3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Mouse anti rabbit Light chain specific
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 130? kDa Non-specific: many kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
Nedd4L shRNA knockdown which we know is working well.
Positive control
Can't tell which one is specific.
Additional data
Additional Notes
In parallel we tried the rabbit monoclonal to Nedd4L (ab131167) and that worked very well in western blot.
Abcam response
Thank you for sharing this data with us. ab46521 has previously been tested by western blot on a transfected human cell line. However, based on this abreview ab46521 does not recognise Nedd4L, under these experimental conditions. Abcam would welcome feedback from other researchers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Wei Cui
Verified customer
提交于 Mar 11 2015