Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (CNS Tumour)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
CNS Tumour
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Diluent: NHS
Dilution
1/2000
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used:
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Abcam response
Thank you for sharing your experience on using ab95153 in an untested species, human. We are happy to hear that the antibody has given satisfactory results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jim Manavis
Verified customer
提交于 Aug 16 2016