Application
Western blot
Sample
Human Cell lysate - whole cell (fibroblast)
Loading amount
100 µg
Specification
fibroblast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: AntiMouse HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 140 kDa Non-specific: 60 kDa
Positive control
human fibroblast
Negative control
zebrafish brain and zebrafish whole embryo lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 15 2012