重组Anti-NAT1抗体[EPR3221(2)] (ab109114)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3221(2)] to NAT1
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-NAT1抗体[EPR3221(2)]
参阅全部 NAT1 一抗 -
描述
兔单克隆抗体[EPR3221(2)] to NAT1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra)more details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- U937, fetal liver, LnCaP, human bladder, and HT-1376 cell lysates and LN-CAP cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3221(2) -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109114于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
|
|
ICC/IF |
1/100 - 1/250.
|
|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 34 kDa. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
靶标
-
功能
Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. -
序列相似性
Belongs to the arylamine N-acetyltransferase family. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 9 Human
- Omim: 108345 Human
- SwissProt: P18440 Human
- Unigene: 2 Human
- Unigene: 591847 Human
-
别名
- AAC1 antibody
- ARY1_HUMAN antibody
- Arylamide acetylase 1 antibody
see all
图片
-
All lanes : Anti-NAT1 antibody [EPR3221(2)] (ab109114) at 1/1000 dilution
Lane 1 : U937 cell lysate
Lane 2 : Fetal liver cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : Human bladder cell lysate
Lane 5 : HT-1376 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa -
Flow Cytometry analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling NAT1 with purified ab109114 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
Immunofluorescent staining of LN-CAP cells using anti-NAT1 at 1/100 (ab109114).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab109114 被引用在 5 文献中.
- Salazar-González RA et al. Arylamine N-Acetyltransferase 1 Activity is Regulated by the Protein Acetylation Status. Front Pharmacol 13:797469 (2022). PubMed: 35153780
- McAleese CE et al. Arylamine N-acetyltransferase 1 deficiency inhibits drug-induced cell death in breast cancer cells: switch from cytochrome C-dependent apoptosis to necroptosis. Breast Cancer Res Treat 195:223-236 (2022). PubMed: 35918499
- Salazar-González RA et al. Human arylamine N-acetyltransferase 2 genotype-dependent protein expression in cryopreserved human hepatocytes. Sci Rep 10:7566 (2020). PubMed: 32372066
- Stepp MW et al. N-Acetyltransferase 1 Knockout Elevates Acetyl Coenzyme A Levels and Reduces Anchorage-Independent Growth in Human Breast Cancer Cell Lines. J Oncol 2019:3860426 (2019). PubMed: 31531019
- Hernández-González O et al. Genetic polymorphisms of arylamine N-acetyltransferases 1 and 2 and the likelihood of developing pediatric acute lymphoblastic leukemia. Leuk Lymphoma 59:1968-1975 (2018). PubMed: 29214875