Application
Western blot
Sample
Human Cell lysate - whole cell (Lec2)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Specification
Lec2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s)
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: ScanLater Goat Anti-Rabbit Eu-labeled Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Europium
Host species: Goat
Clonality: Polyclonal
Conjugation: Europium
Detection
Detection method
Fluorescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 82 kDa
Positive control
Recombinant Protein from Novus with Tag
Additional data
Additional Notes
Lane 1 - Ladder
Lane 2 - PC 12.5 ng
Lane 3 - PC 25 ng
Lane 4 - PC 50 ng
Lane 5 - NAGLU Transfected Cell
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 31 2017