Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% triton-x
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: block
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 17 2010