Application
Western blot
Sample
Human Cell lysate - whole cell (A549 Lung adenocarcinoma cell line)
Loading amount
20 µg
Specification
A549 Lung adenocarcinoma cell line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 100-150 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Zahra Abdalla
Verified customer
提交于 Dec 20 2012