Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
120 µg
Specification
Hela
Treatment
10ng/ml IL6 for 30 minutes
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: same as blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit HRP-conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Additional data
Abcam response
Thank you for submitting this review. This antibody has not been previously tested in western blotting. The reviewer did not see any bands on their blot. ab59202 may not be suitable for western blotting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 02 2008