Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic (E18.5) cardiomyocyte)
Specification
Embryonic (E18.5) cardiomyocyte
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Dilution
1/140
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum 10% BSA 0.1% Tx100 PBS
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200
Additional data
Additional Notes
4% paraformaldehyde 10 minutes at room temperature.
Blocking and antibody incubation buffer : 1% goat serum 10% BSA 0.1% Tx100 PBS
Blocking and antibody incubation buffer : 1% goat serum 10% BSA 0.1% Tx100 PBS
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提交于 Jun 18 2012