Application
Western blot
Sample
Rat Tissue lysate - whole (Ileon)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
Ileon
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS Tween 0,5% Lait 5%
Dilution
1/200
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Clarity
Exposure
1 minute(s) and 40 second(s)
Positive control
ab11082 used on the same sample and same western blot run (cf picture)
Additional data
Additional Notes
Lane 0: Ladder
Lane 1: Rat ileon sample 1
Lane 2: Rat ileon sample 2
Lane 3: Rat ileon sample 3
Lane 1-3 second mb: same sample used with ab92721
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 30 2015