Anti-Myosin Light Chain 2抗体[AT3B2] (ab89594)
Key features and details
- Mouse monoclonal [AT3B2] to Myosin Light Chain 2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Rat, Human
- Isotype: IgG2b
概述
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产品名称
Anti-Myosin Light Chain 2抗体[AT3B2]
参阅全部 Myosin Light Chain 2 一抗 -
描述
小鼠单克隆抗体[AT3B2] to Myosin Light Chain 2 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant full length protein corresponding to Human Myosin Light Chain 2 aa 1-166.
Database link: P10916 -
阳性对照
- Rat heart extracts. Flow Cyt (Intra).:A431 cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 11th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.2% Sodium azide
Constituents: PBS, 10% Glycerol -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
AT3B2 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89594于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
靶标
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疾病相关
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
序列相似性
Contains 3 EF-hand domains. -
翻译后修饰
N-terminus is methylated by METTL11A/NTM1. - Information by UniProt
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数据库链接
- Entrez Gene: 4633 Human
- Entrez Gene: 363925 Rat
- Omim: 160781 Human
- SwissProt: P10916 Human
- SwissProt: P08733 Rat
- Unigene: 75535 Human
- Unigene: 37176 Rat
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别名
- Cardiac myosin light chain-2 antibody
- Cardiac ventricular myosin light chain 2 antibody
- CMH10 antibody
see all
图片
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Overlay histogram showing A431 cells stained with ab89594 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89594, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This image was generated using the ascites version of the product.
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Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/1000 dilution + rat heart extracts at 60 µg
Secondary
goat anti-mouse HRP
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 19 kDaThis image was generated using the ascites version of the product.
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Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/2000 dilution + Rabbit heart tissue lysate at 10 µg
Secondary
HRP conjugated goat anti-mouse at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 1 minuteThis image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab89594 被引用在 6 文献中.
- Pierre M et al. NaV1.5 knockout in iPSCs: a novel approach to study NaV1.5 variants in a human cardiomyocyte environment. Sci Rep 11:17168 (2021). PubMed: 34433864
- Sapp V et al. Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity. Sci Rep 11:13866 (2021). PubMed: 34230586
- Mourino-Alvarez L et al. A comprehensive study of calcific aortic stenosis: from rabbit to human samples. Dis Model Mech 11:N/A (2018). PubMed: 29752279
- Acharya BR et al. A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress. Dev Cell 47:439-452.e6 (2018). PubMed: 30318244
- Russo V et al. Porous, Ventricular Extracellular Matrix-Derived Foams as a Platform for Cardiac Cell Culture. Biores Open Access 4:374-88 (2015). PubMed: 26487982
- Beveridge RD et al. The leukemia-associated Rho guanine nucleotide exchange factor LARG is required for efficient replication stress signaling. Cell Cycle 13:3450-9 (2014). Human . PubMed: 25485589