Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle)
Loading amount
20 µg
Specification
Muscle
Gel Running Conditions
Reduced Denaturing (12% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST plus 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Dako Cytomation P0260
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 25 kDa Non-specific: 37, 64, 74, 95 kDa
Positive control
Muscle
Additional data
Additional Notes
Myogenin antibody ab1835 is specific for myogenin but also cross-reacts with a number of other muscle proteins
Abcam response
Many thanks for your WB data on ab1835 with mouse muscle tissue lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 Mar 24 2010