Anti-Myeloperoxidase抗体(ab45977)
Key features and details
- Rabbit polyclonal to Myeloperoxidase
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Myeloperoxidase抗体
参阅全部 Myeloperoxidase 一抗 -
描述
兔多克隆抗体to Myeloperoxidase -
宿主
Rabbit -
特异性
ab45977 has been batch tested using human lysates/tissues only. Some customers have successfully used ab45977 with rat. Please contact Abcam Scientific Support for more information. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat, Common marmoset -
免疫原
Synthetic peptide corresponding to Human Myeloperoxidase aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab45976) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45977于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
Use a concentration of 5 - 10 µg/ml.
|
WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
|
IHC-P | (11) |
Use a concentration of 1 µg/ml.
|
说明 |
---|
ICC/IF
Use a concentration of 5 - 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
IHC-P
Use a concentration of 1 µg/ml. |
靶标
-
功能
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
疾病相关
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
序列相似性
Belongs to the peroxidase family. XPO subfamily. -
细胞定位
Lysosome. - Information by UniProt
-
数据库链接
- Entrez Gene: 4353 Human
- Entrez Gene: 303413 Rat
- Omim: 606989 Human
- SwissProt: P05164 Human
- Unigene: 458272 Human
-
别名
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
see all
图片
-
ab45977 stained in HL60 cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45977 at 5µg/ml and ab72791 (Mouse monoclonal to alpha Tubulin - Loading Control) at 1ug/ml overnight at +4°C. The secondary antibodies used were ab150081 used at 1 ug/ml (colored green) and ab150116 (pseudo-colored red) used at 2ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
-
ab45977 (1/50) staining Myeloperoxidase in paraffin-embbeded human spleen tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview.
-
Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab45977 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
-
IHC image of 45977 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45977, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 12 kDa, 35 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesThe mature myeloperoxidase protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). Our immunogen sequence is within the myeloperoxidase light chain. In HL60 cells, ab45977 detects bands at approximately 84-kDa, corresponding to the expected MW of full-length Myeloperoxidase protein, and at 12-kDa, corresponding to the expected MW of myeloperoxidase light chain.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (74)
ab45977 被引用在 74 文献中.
- Kawasaki M et al. Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation. Sci Rep 11:2972 (2021). PubMed: 33536523
- Kato Y et al. Effects of Thrombomodulin in Reducing Lethality and Suppressing Neutrophil Extracellular Trap Formation in the Lungs and Liver in a Lipopolysaccharide-Induced Murine Septic Shock Model. Int J Mol Sci 22:N/A (2021). PubMed: 34066510
- Le A et al. MiR-144-induced KLF2 inhibition and NF-kappaB/CXCR1 activation promote neutrophil extracellular trap-induced transfusion-related acute lung injury. J Cell Mol Med 25:6511-6523 (2021). PubMed: 34120407
- Goonetilleke M et al. Addressing the liver progenitor cell response and hepatic oxidative stress in experimental non-alcoholic fatty liver disease/non-alcoholic steatohepatitis using amniotic epithelial cells. Stem Cell Res Ther 12:429 (2021). PubMed: 34321089
- Neehus AL et al. Impaired respiratory burst contributes to infections in PKCδ-deficient patients. J Exp Med 218:N/A (2021). PubMed: 34264265