Anti-MZF1抗体(ab64866)
Key features and details
- Rabbit polyclonal to MZF1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MZF1抗体 -
描述
兔多克隆抗体to MZF1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MZF1 (N terminal).
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阳性对照
- Extracts from 293 and K562 cells; human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64866于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
Use a concentration of 1 - 5 µg/ml.
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WB | (3) |
1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
|
IHC-P |
1/50 - 1/100.
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说明 |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). |
IHC-P
1/50 - 1/100. |
靶标
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功能
Binds to target promoter DNA and functions as trancription regulator. Regulates transcription from the PADI1 and CDH2 promoter. May be one regulator of transcriptional events during hemopoietic development. -
组织特异性
Preferentially expressed in differentiating myeloid cells. Detected in osteoblasts. -
序列相似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 13 C2H2-type zinc fingers.
Contains 1 SCAN box domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7593 Human
- Omim: 194550 Human
- SwissProt: P28698 Human
- Unigene: 399810 Human
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别名
- Myeloid zinc finger 1 antibody
- MZF 1 antibody
- MZF-1 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab64866 at a dilution of 1/50 - 1/100. Left hand panel - no immunising peptide; right hand panel - immunising peptide.
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All lanes : Anti-MZF1 antibody (ab64866) at 1/500 dilution
Lane 1 : Extracts from 293 cells with no immunising peptide
Lane 2 : Extracts from K562 cells with no immunising peptide
Lane 3 : Extracts from K562 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 82 kDa
Observed band size: 82 kDa -
ICC/IF image of ab64866 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64866, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab64866 被引用在 13 文献中.
- Qi Z et al. MZF1 Transcriptionally Activated MicroRNA-328-3p Suppresses the Malignancy of Stomach Adenocarcinoma via Inhibiting CD44. J Immunol Res 2022:5819295 (2022). PubMed: 35669102
- Zhang Z et al. Kinase GSK3ß functions as a suppressor in colorectal carcinoma through the FTO-mediated MZF1/c-Myc axis. J Cell Mol Med 25:2655-2665 (2021). PubMed: 33533172
- Fang E et al. Therapeutic targeting of YY1/MZF1 axis by MZF1-uPEP inhibits aerobic glycolysis and neuroblastoma progression. Theranostics 10:1555-1571 (2020). PubMed: 32042322
- Cao Y et al. Lestaurtinib potentiates TRAIL-induced apoptosis in glioma via CHOP-dependent DR5 induction. J Cell Mol Med 24:7829-7840 (2020). PubMed: 32441887
- Podolsky MJ et al. Age-dependent regulation of cell-mediated collagen turnover. JCI Insight 5:N/A (2020). PubMed: 32315288