Application
ELISA
Sample
Human Cell (brain)
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type
Indirect
Other product details
Dilution
1/10000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/500
Additional data
Additional Notes
Following the incubation with biotinylated anti-rabbit, the plate was incubated with HRP-conjugated streptavidin dilute 1:500 for 20 min.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Rachel Barker
Verified customer
提交于 Nov 06 2012