Application
Western blot
Sample
Mouse Tissue lysate - whole (liver and brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-Mouse IgG -HRP linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Negative control
liver
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 21, 17, 14 kDa Non-specific: 40, 50 kDa
Positive control
brain
Additional data
Additional Notes
several unspecific band observed... different between liver and brain.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 17 2017