Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal Cord)
Specification
Spinal Cord
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Vector Horse anti-rat-Biotinylated
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
These animals were transcardially perfused with cold PB followed by 4% formaldehyde in PBS. Tissue was transfered to 30% sucrose for three days, mounted in OCT and stored at -80 until used. 20 um sections were used.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Nancy Nutile-McMenemy
Verified customer
提交于 Mar 30 2007