Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
25 µg
Specification
293T
Treatment
Cells transfected with K8-Myc-tag
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Amersham-HRP sheep anti-rabbit
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 56 kDa
Positive control
overexpression of K8-myc-tag protein line1
control cells line2
control cells line2
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 03 2012