HRP Anti-Myc tag抗体[Myc.A7] (ab173830)
Key features and details
- HRP Mouse monoclonal [Myc.A7] to Myc tag
- Suitable for: WB
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
HRP Anti-Myc tag抗体[Myc.A7]
参阅全部 Myc tag 一抗 -
描述
HRP小鼠单克隆抗体[Myc.A7] to Myc tag -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WBmore details -
免疫原
Synthetic peptide within Human Myc tag aa 410-419 (C terminal). The exact sequence is proprietary.
Sequence:EQKLISEEDL
Database link: P01106 -
阳性对照
- E. coli lysate containing a multi-epitope tagged protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
Myc.A7 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Related Products
- Recombinant human c-Myc (mutated T73A) protein (ab135024)
- Recombinant human c-Myc protein (Active) (ab169901)
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- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- Recombinant Human c-Myc protein (ab84132)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173830于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 1 kDa.
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说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 1 kDa. |
靶标
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相关性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
细胞定位
Nuclear -
别名
- c-myc tag antibody
- Myc Epitope Tag antibody
图片
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All lanes : HRP Anti-Myc tag antibody [Myc.A7] (ab173830) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 1 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0500000007450581 µg
Lane 6 : E. coli lysate containing a multi-epitope tagged protein at 0.025000000372529 µg
Developed using the ECL technique.
Predicted band size: 1 kDaNote: predicted MW is for the Myc tag only.
数据表及文件
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Datasheet download
文献 (0)
ab173830 尚未被引用在任何文献中。