Application
Western blot
Sample
Escherichia coli Recombinant protein (Expressed in DH10B cell)
Loading amount
0.5 µg
Specification
Expressed in DH10B cell
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
45 minute(s) · Diluent: TBST+5%Milk
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 44 kDa
Additional data
Additional Notes
The MW of the target protein is ~44kD.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 17 2010