Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophages cell line)
Loading amount
50 µg
Specification
macrophages cell line
Treatment
LPS
Gel Running Conditions
Reduced Denaturing (10-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: sc-2030
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 33 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Varkha Agrawal
Verified customer
提交于 Sep 10 2012