Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Intact cerebellum)
Specification
Intact cerebellum
Fixative
Formaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Dilution
1/20
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
The antibody was applied on 2% PFA-fixed, 16 µm thick sections of brown ghost knifefish brain. No nuclear staining was observed, but some clear labeling in what looks like radial glia in a known active proliferation zone of the cerebellum.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Ruxandra Sirbulescu
Verified customer
提交于 Sep 28 2011