Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
30 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 50mMTris,pH8.0,50mMKCl,1mMEDTA,5%glycero
Secondary antibody
Name
Non-Abcam antibody was used: IR Dye 800 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000
Detection
Detection method
Odyssey® scanner
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: - kDa Non-specific: 42 kDa
Positive control
Whole Cell Extract
Negative control
-
Additional data
Additional Notes
Picture legend
Mouse embryonic fibroblasts Whole Cell Extract (30 µg)
Mouse embryonic fibroblasts Whole Cell Extract (30 µg)
Abcam response
Thank you for your feedback.
The results suggest this antibody is not suitable for Mouse. To our knowledge, this is the first time the anti-MUS81 ab14387 is tested in Mouse.
Thanks to this feedback, the product datasheet has been updated with the information that ab14387 is not suitable for Mouse samples.
The results suggest this antibody is not suitable for Mouse. To our knowledge, this is the first time the anti-MUS81 ab14387 is tested in Mouse.
Thanks to this feedback, the product datasheet has been updated with the information that ab14387 is not suitable for Mouse samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 13 2011